Journal: Cells

Article Title: Antibody–Drug Conjugate Made of Zoledronic Acid and the Anti-CD30 Brentuximab–Vedotin Exert Anti-Lymphoma and Immunostimulating Effects

doi: 10.3390/cells13100862

Figure Lengend Snippet: Reduction of HL-cell growth in the presence of Bre–Ved–ZA compared to Bre–Ved. ( A ) Images of KMH2 cell cultures, without or with 2 µg/mL of Bre–Ved–ZA or Bre–Ved, taken at 24, 48, and 72 h with the CELLCYTE X TM imaging recorder are shown. Magnification 50×; one well/each time point is shown. Bar: 100 µm. ( B ) Cell area calculated by image analysis (CELLCYTE Studio software) on images of proliferating KMH2 cell cultures, without or with Bre–Ved–ZA or Bre–Ved (2.0 µg/mL or 0.2 µg/mL) taken at 24, 48, 72 h, expressed as mm 2 . ( C ) Cell area, calculated as in ( A ), of L428 or L540 clusters at 24, 48, and 72 h expressed in mm 2 . Results are the mean ± SD of three independent experiments from 4 replicated wells. *** p < 0.001 of Bre–Ved–ZA vs. Bre–Ved at 2.0 µg/mL).

Article Snippet: Images of proliferating HL-cells were taken at 24, 48, and 72 h with the CELLCYTE X TM imaging recorder (Cytena, Breisgau, Germany); the cell area was calculated by image analysis using the CELLCYTE Studio software ( https://www.cytena.com/new-cellcyte-x-software-january-2022/ ) and expressed as mm 2 (a representative example for the software mask applied at 24 h is shown in ).

Techniques: Imaging, Software

Journal: Cells

Article Title: Antibody–Drug Conjugate Made of Zoledronic Acid and the Anti-CD30 Brentuximab–Vedotin Exert Anti-Lymphoma and Immunostimulating Effects

doi: 10.3390/cells13100862

Figure Lengend Snippet: HL-cell death induced by Bre–Ved–ZA ADC compared to Bre–Ved. ( A ) Images of KMH2 cells stained with C.LIVE Tox green fluorescent probe (20 nM) and cultured without or with 2 µg/mL or 0.2 µg/mL Bre–Ved–ZA or Bre–Ved, analyzed at 24, 48, and 72 h with the CELLCYTE X TM imaging recorder. Magnification 50×; one representative well/time point is shown. Bar: 100 µm. ( B ) Fluorescence intensity measured with the CELLCYTE Studio software on images of KMH2 cell cultures, without or with Bre–Ved–ZA or Bre–Ved (2 µg/mL or 0.2 µg/mL) taken at 24, 48, and 72 h, and expressed in arbitrary units (a.u.). ( C ) Fluorescence intensity measured as in A on L428 and L540 cell lines cultured with or without Bre–Ved–ZA or Bre–Ved for the indicated time points (a.u.). Results are the mean ± SD of three independent experiments from 4 replicated wells. *** p > 0.001 of Bre–Ved–ZA vs. Bre–Ved at 2.0 µg/mL.

Article Snippet: Images of proliferating HL-cells were taken at 24, 48, and 72 h with the CELLCYTE X TM imaging recorder (Cytena, Breisgau, Germany); the cell area was calculated by image analysis using the CELLCYTE Studio software ( https://www.cytena.com/new-cellcyte-x-software-january-2022/ ) and expressed as mm 2 (a representative example for the software mask applied at 24 h is shown in ).

Techniques: Staining, Cell Culture, Imaging, Fluorescence, Software

Journal: International Journal of Molecular Sciences

Article Title: New Insights on the Effects of Krill Oil Supplementation, a High-Fat Diet, and Aging on Hippocampal-Dependent Memory, Neuroinflammation, Synaptic Density, and Neurogenesis

doi: 10.3390/ijms252111554

Figure Lengend Snippet: Comparison of stereological analysis of microglia between dietary groups in the hippocampus and cortex. ( A ) Quantification of microglia counts in the rat cortex, dentate gyrus and total combined. Error bars represent SEM. n = 4–6 per group. CON: control diet; CONKO: control diet with krill oil supplementation; HF: high-fat diet; HFKO: high-fat diet with krill oil supplementation. ( B ) Representative image of microglia at 6300× magnification. Ionized calcium-binding adaptor molecule 1 (Iba-1) antibody, DAB substrate, and cresyl violet staining. StereoInvestigator dissector box and markers shown.

Article Snippet: Quantification of microglia in the hippocampus and cortex was assessed with StereoInvestigator imaging software (Version 2022.2.1, MBF Bioscience, Williston, VT, USA), an optical fractionator probe, and systematic random sampling (SRS) and reported as the estimated population mean using section thickness.

Techniques: Comparison, Control, Binding Assay, Staining

Journal: International Journal of Environmental Research and Public Health

Article Title: Volumetric Assessment of Apical Periodontitis Using Cone-Beam Computed Tomography—A Systematic Review

doi: 10.3390/ijerph20042940

Figure Lengend Snippet: The most important parameters of the CBCT apparatus are from included studies.

Article Snippet: Machut et al. [ ] 2022 , CS 3D Imaging v3.5.18 Software (Carestream Health Inc., Trophy, Croissy-Beaubourg, France) , - , - , - , - , - , - , - , ITK-SNAP.

Techniques: Software, Imaging